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The principle and Procedure of Polyacrylamide Gel Electrophoresis (SDS-PAGE)

sds page in dna extraction

SDS-PAGE (sodium dodecyl sulfate – polyacrylamide gel electrophoresis) is a technique used to separate the proteins according to their masses.  Separation of macromolecules under the influence of the charge is called electrophoresis. The gel used in SDA-PAGE is polyacrylamide and agent which is used to linearize the proteins is SDS. Hence the name SDS-PAGE. Principle …