• Home
  • Trends in Biotechnology
  • About Us
  • Techniques

How Biotech

Techniques

Isotopic Labelling and NMR Analysis of Proteins

By Ali
Techniques

Plasmid DNA Isolation-Basic Principle & Procedure

By Ali
Techniques

Gram Staining – Principle , Procedure , Function of Reagents

By Ali
Techniques

CRISPR Cas9 Gene Editing Mechanism

By Ali
Techniques

Agarose Gel Electrophoresis for DNA

By Ali
Techniques

Cancer- Causes and Development

By Ali
Techniques

HIV/AIDS cure antiretroviral therapy (ART)tre-recombinases , zink finger nucleases (ZFNs)

By Ali
Techniques

The principle and Procedure of Polyacrylamide Gel Electrophoresis (SDS-PAGE)

By Ali
Techniques

HIV Structure and Genome

By Ali
Techniques

AIDS global epidemic transmission challenges and cure

By Ali
spectrometer for NMR analysis
Techniques

Isotopic Labelling and NMR Analysis of Proteins

By Ali on Monday, June 17, 2019

What is Isotopic labelling? Isotopic labelling is defined as a methodology that is widely used to introduce less common isotope of carbon, hydrogen, nitrogen and sometimes oxygen into the proteins or polypeptide chains [1]. All isotopes of an element exhibit the same chemical properties because of the same atomic number but different physical properties due …

Continue Reading
0
Plasmid DNA Isolation
Techniques

Plasmid DNA Isolation-Basic Principle & Procedure

By Ali on Wednesday, June 5, 2019

Introduction Generally, Plasmid purification from the culture of bacterial cells has a similar strategy like preparation of total cell DNA. A cell culture having plasmids is grown in a medium to prepare cell extract. The RNA and different proteins are removed from the whole extract by using different techniques. After that, precipitation of DNA is …

Continue Reading
0
Gram Staining
Techniques

Gram Staining – Principle , Procedure , Function of Reagents

By Ali on Wednesday, January 30, 2019

Gram staining is basically a staining technique which is broadly used in microbial and molecular biology . This technique was developed by a Danish bacteriologist Hans Christian Gram in 1884. Gram staining is used for the identification and differentiation of different types of bacteria. It differentiates Gram positive bacteria and Gram negative bacteria on the …

Continue Reading
0
crispr cas9
Techniques

CRISPR Cas9 Gene Editing Mechanism

By Ali on Sunday, January 20, 2019

Structure of Cas9 Cas9 is the functional unit of CRISPR Cas9 system that locates the target gene by sliding over the DNA and when it finds the protospacer adjacent motif (PAM) sequence, it performs its catalytic activity and produces nicks or DSB in the DNA leading to the alteration in the gene sequence. Mostly these …

Continue Reading
0

Posts navigation

1 2 3 4 Next →
Theme by Scissor Themes Proudly powered by WordPress