What is Isotopic labelling? Isotopic labelling is defined as a methodology that is widely used to introduce less common isotope of carbon, hydrogen, nitrogen and sometimes oxygen into the proteins or polypeptide chains [1]. All isotopes of an element exhibit the same chemical properties because of the same atomic number but different physical properties due …
Introduction Generally, Plasmid purification from the culture of bacterial cells has a similar strategy like preparation of total cell DNA. A cell culture having plasmids is grown in a medium to prepare cell extract. The RNA and different proteins are removed from the whole extract by using different techniques. After that, precipitation of DNA is …
Gram staining is basically a staining technique which is broadly used in microbial and molecular biology . This technique was developed by a Danish bacteriologist Hans Christian Gram in 1884. Gram staining is used for the identification and differentiation of different types of bacteria. It differentiates Gram positive bacteria and Gram negative bacteria on the …
Structure of Cas9 Cas9 is the functional unit of CRISPR Cas9 system that locates the target gene by sliding over the DNA and when it finds the protospacer adjacent motif (PAM) sequence, it performs its catalytic activity and produces nicks or DSB in the DNA leading to the alteration in the gene sequence. Mostly these …
